Department of Ophthalmology Picture Department of Ophthalmology Picture Department of Ophthalmology Picture Department of Ophthalmology Picture Department of Ophthalmology Picture Department of Ophthalmology Picture Department of Ophthalmology Picture

Research Faculty

Shannon E. Boye, Ph.D.

Shannon Boye
Shannon Boye, Ph.D.

Dr. Shannon Boye received her B.S. in marine biology with a minor in chemistry from Fairleigh Dickinson University in 2001. Following a National Science Foundation-sponsored, undergraduate internship at the Whitney Marine laboratory, she decided to pursue a career in biomedical research. In 2006, she received her Ph.D. in Neuroscience at the University of Florida. Her thesis work involved the use viral vectors for the treatment of retinal disease, specifically Leber Congenital Amaurosis-1 (LCA1). After receiving her doctorate, Dr. Boye accepted a postdoctoral position in the laboratory of Dr. William Hauswirth. For two years, she both expanded upon her graduate work and began using AAV-based gene therapy to address other inherited retinal disorders. In 2008, Dr. Boye was appointed as research assistant professor. The retinal disorders she currently studies include, but are not limited to, Leber congenital amaurosis (LCA1, LCA2 and CEP290-LCA) and Usher syndrome. Because of its importance to human vision, much of Dr. Boye’s work focuses on cone photoreceptor-targeted therapy. Her other major interest is optimizing AAV vectors to successfully treat diseases which involve mutations in large genes (something recently considered outside the scope of standard AAV gene therapy). Her membership in professional societies includes the Association for Research in Vision and Ophthalmology (ARVO) and the American Society of Gene and Cell Therapy (ASGCT). She currently serves as Co-PI or Co-Investigator on several federally as well as privately funded grants. Dr. Boye is fortunate to share her passion for science with her husband, Sanford Boye. If ever they are both missing from the lab, rest assured they are on a boat somewhere in the middle of the Gulf of Mexico doing their second favorite thing… grouper fishing!

Dr. Boye has authored numerous peer-reviewed manuscripts and textbook chapters which are listed below:

Manuscripts:

  1. Shannon E. Boye, John J. Alexander, Sanford L. Boye, Clark D. Witherspoon, Kristen J. Sandefer, Thomas J. Conlon, Kirsten Erger, Vince A. Chiodo, Mark E. Clark, Christopher A. Girkin, William W. Hauswirth, Paul D. Gamlin. The human rhodopsin kinase promoter in an AAV5 vector confers rod and cone specific expression in the primate retina. Submitted to Gene Therapy (2012).

  2. Hong Yu, Rajeshwari D. Koilkonda, Tsung-Han Chou, Vittorio Porciatti, Sacide Ozdemir, Vince Chiodo, Sanford Boye, Shannon Boye, William W. Hauswirth, Nicholas Muzycyka, Alfred S. Lewin, and John Guy. Mitochondrial gene complementation of the NADH Ubiquinone oxidoreductase mediated by an MTS modification to adeno-associated virus. Proc Natl Acad Sci U S A. In Press.

  3. Li Jiang, Houbin Zhang, Alexander Dizhoor, Shannon E. Boye, William W. Hauswirth, Jeanne Frederick, Wolfgang Baehr. RNA interference-based gene therapy in a dominant retinitis pigmentosa mouse model. (2011) Proc Natl Acad Sci U S A. Sep 9;52(10):7098-108.

  4. Sanford L. Boye, Thomas Conlon, Kirsten Erger, Renee Ryals, Andy Neeley, Travis Cossette, Jijing Pang, Frank M. Dyka, William W. Hauswirth, Shannon E. Boye. (2011) Long term preservation of cone photoreceptors and restoration of cone function by gene therapy in the guanylate cyclase-1 knockout (GC1KO) mouse. IOVS. Sep 9;52(10):7098-108

  5. M. Mehdi Doroudchi, Kenneth P. Greenberg, Jianwen Liu, Kimberly A. Silka, Edward S. Boyden, Jennifer A. Lockridge, A. Cyrus Arman, Ramesh Janani, Shannon E. Boye, Sanford L. Boye, Gabriel M. Gordon, Benjamin C. Matteo, Alapakkam P. Sampath, William W. Hauswirth, Alan Horsager. (2011) Virally-Delivered Channelrhodopsin-2 Safely and Effectively Restores Visual Function in Multiple Mouse Models of Blindness. Mol Ther. Jul;19(7):1220-9.

  6. Renee C. Ryals, Sanford L. Boye, William W. Hauswirth, Shannon E. Boye. (2011) Quantifying transduction efficiencies of unmodified and tyrosine capsid mutant AAV vectors in vitro using two ocular cell lines. Molecular Vision. Apr 29;17:1090-102.

  7. Jijing Pang JJ, Xufeng Dai, Shannon E. Boye, Ilaria Barone, Sanford L Boye, Song Mao, Drew Everhart, Astra Dinculescu, Li Liu, Yumiko Umino, Bo Lei, Bo Chang, Robert Barlow, Enrica Strettoi, William W Hauswirth. (2011) Long-term retinal function and structure rescue using capsid mutant AAV8 vector in the rd10 mouse, a model of recessive retinitis pigmentosa. Mol Ther. Feb;19(2):234-42.

  8. Shannon E. Boye, Sanford L. Boye, Jijing Pang, Renee Ryals, Drew Everhart, Yumiko Umino, Andy W. Neeley, Joseph Besharse, Robert Barlow, William W. Hauswirth. (2010) Functional and behavioral restoration of vision by gene therapy in a mouse model of Leber congenital amaurosis-1 (LCA1). PLoS ONE. 5: e11306.

  9. William A. Beltran, Sanford L. Boye, Shannon E. Boye, Vince A. Chiodo, Gregory M. Acland, Alfred S. Lewin, William W. Hauswirth, Gustavo D. Aguirre. (2010) rAAV2/5 gene-targeting to rods: dose-dependent efficiency and complications associated with different promoters. Gene Therapy. Sep;17(9):1162-74.

  10. Jijing Pang*, Shannon Boye*, Bo Lei, Sanford L. Boye, Drew Everhart, Yumiko Umino, Baerbel Rohrer, John Alexander, Jie Li, Bo Chang, Robert Barlow, and William W. Hauswirth. (2010) Self-complementary AAV-mediated gene therapy restores cone function and prevents cone degeneration in two models of Rpe65 deficiency. Gene Therapy. 17:815-26. *equal contribution

  11. Roman AJ, Boye SL, Aleman TS, Pang JJ, McDowell JH, Boye SE, Cideciyan AV, Jacobson SG, Hauswirth WW. (2007) Electroretinographic analyses of Rpe65-mutant rd12 mice: developing an in vivo bioassay for human gene therapy trials of Leber congenital amaurosis. Mol Vis. 13:1701-10.

  12. Haire SE, Pang J, Boye SL, Sokal I, Craft CM, Palczewski K, Hauswirth WW, Semple-Rowland SL. (2006) Light-driven cone arrestin translocation in cones of postnatal guanylate cyclase-1 knockout mouse retina treated with AAV-GC1. Invest Ophthalmol Vis Sci. Sep;47(9):3745-53

  13. Williams ML, Coleman JE, Haire SE, Aleman TS, Cideciyan AV, Sokal I, Palczewski K, Jacobson SG, Semple-Rowland SL. (2006) Lentiviral Expression of Retinal Guanylate Cyclase-1 (RetGC1) Restores Vision in an Avian Model of Childhood Blindness. PLoS Med. Jun;3(6):e201.

  14. Jijing Pang, Mei Cheng, Shannon E. Haire, Vicente Planelles and Janet C. Blanks. (2006) Efficiency of lentiviral transduction during development in normal and rd mice. Mol Vis. Jul 11;12:756-67

Book Chapters:

  1. Title: Mouse Visual System
    Editors: Chalupa and Williams
    Publisher: MIT Press
    Chapter Title: AAV Gene Therapy in Mouse Models of Retinal Degeneration
    Authors: SE Boye , SL Boye, WW Hauswirth
    2008

  2. Title: A Guide to Human Gene Therapy
    Editors: Herzog and Zolotukhin
    Publisher: World Scientific
    Chapter Title: Retinal Diseases
    Authors: Shannon E Boye, Sanford L Boye, William W. Hauswirth
    2009

  3. Title: Regenerative Medicine
    Editors: Robert C. Fisher and Edward W. Scott
    Publisher: Jones and Bartlett
    Chapter Title: Retina
    Authors: Hilda Petrs-Silva, Shannon Boye and William W. Hauswirth